楊瑞麗     施用暉^[1]    李武  岳鵬  樂(lè )國偉²

江南大學(xué)食品學(xué)院，無(wú)錫  214122

摘要：目的  探討抗氧化劑硫辛酸(LA)對高脂膳食小鼠血糖血脂代謝和抗氧化能力的影響。方法  24只C57BL/6雄性小鼠，隨機分為3組：即對照組(正常飼料)，高脂組(高脂飼料)，LA組(高脂飼料+0.1%LA)，6周后測定空腹血糖（FPG）、胰島素(FINS)、三酰甘油(TG)、總膽固醇(TC)等糖脂代謝指標以及丙二醛(MDA)、超氧化物歧化酶(SOD)和總抗氧化能力(TAC)等抗氧化指標，同時(shí)用Affymetrix MOE430A 基因芯片分析肝臟抗氧化、糖脂代謝相關(guān)功能基因的表達。結果  與高脂組相比，LA組MDA含量顯著(zhù)降低(P<0.05)，TAC明顯升高。LA顯著(zhù)降低血漿TG、TC、LDL–C和FPG、FINS的水平，MDA含量與HDL-C/TC(r=-0.47，P<0.05)和胰島素抵抗指數(r=0.74，P<0.05)顯著(zhù)相關(guān)。硫辛酸改變高脂組自由基/氧化應激、糖脂代謝相關(guān)通路大部分響應基因的表達水平。結論  高脂引發(fā)氧化－抗氧化系統失衡，硫辛酸通過(guò)直接清除自由基以及恢復自由基/氧化應激通路相關(guān)基因的表達水平解除氧化應激而有助于防治高脂引發(fā)的糖脂代謝紊亂。

關(guān)鍵詞：硫辛酸  氧化應激  基因芯片  糖代謝  脂代謝

中圖分類(lèi)號：R151.2  Q591  Q786                       文獻標識碼：A

 

Effect of lipoic acid on gene expression related to oxidative stress, lipid metabolism and glycometabolism of mice fed a high fat diet

YANG Ruili, SHI Yonghui, LI Wu, YUE Peng, et al.

Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China

 

Abstract：Objective To explore the effect of lipoic acid (LA) on oxidative stress, lipid metabolism and glycometabolism of mice fed with high fat diet (HFD). Methods C57BL/6 mice were randomly assigned to one of three groups (n=8).The control group consumed an ordinary diet. The other two experimental groups were fed with a high fat high diet, high fat plus 0.1% LA. Fasting plasma glucose, insulin, lipid level and antioxidant status were examined after 6 weeks. To investigate the molecular mechanisms underlying the effects of LA, we examined gene expression profiles in liver using the GeneChip microarray system. Results Hyperlipidemia and glycometabolism disorders, accompanied by a depressed antioxidant defense system, were observed in HFD-fed mice. These changes were partially restored in the LA-treated group. There was a correlation between MDA and the ratio of HDL-C/TC(r=-0.47, P<0.05), homeostasis model assessment (HOMA) index(r=0.74, P<0.05). DNA microarray analysis of liver showed that LA ingestion up-regulated the expression of genes related to free-radical scavenger enzymes, ß-oxidation and glycometabolism. Conclusions Treatment with LA improves insulin resistance and hyperlipidemia associated with HFD feeding in mice. The mechanism may involve preventing oxidative stress by scavenging reactive oxygen species (ROS) directly and increasing those of free-radical scavenger enzymes genes expression indirectly.

Key word: lipoic acid, oxidative stress, gene expression, glucose metabolism, lipid metabolism

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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基金項目：國家自然科學(xué)基金項目（No.30571347）

作者簡(jiǎn)介：楊瑞麗，女，博士研究生

1食品科學(xué)與技術(shù)國家重點(diǎn)實(shí)驗室 江南大學(xué)

2通訊作者：樂(lè )國偉，男，教授，博士生導師