青年科學(xué)工作者論壇2007年第6期

《衛生研究》--2007年第六期

論著(zhù)

文章編號：1000-8020（2007）06-0716-03

轉鐵蛋白受體評價(jià)我國育齡期婦女缺鐵性貧血的閾值探討

李劍虹楊麗琛1 樸建華楊曉光1

中國疾病預防控制中心營(yíng)養與食品安全所，北京 100050

摘要： 目的比較中國居民育齡期婦女缺鐵性貧血人群與鐵營(yíng)養狀況正常人群血漿可溶性轉鐵蛋白受體（sTfR）水平，并確定sTfR在評價(jià)缺鐵性貧血時(shí)的適宜臨界值。方法從2002年中國居民營(yíng)養與健康狀況調查采集的靜脈血液樣本中隨機抽取251名鐵營(yíng)養狀況正常的育齡期婦女作為正常對照組，224名缺鐵性貧血的育齡期婦女作為病例組，比較兩組人群sTfR水平，采用ROC曲線(xiàn)法探討其評估缺鐵性貧血的最佳臨界值。結果缺鐵性貧血組與正常對照組血漿sTfR濃度分別為（42.67±20.29）nmol/L和（21.08±6.70）nmol/L，缺鐵性貧血組sTfR水平顯著(zhù)高于正常對照組（P＜0.01）。采用ROC曲線(xiàn)法確定sTfR判斷缺鐵性貧血的最佳臨界點(diǎn)為25 nmol/L，對應的敏感度為87.48%，特異度為80.50%。結論中國育齡期婦女缺鐵性貧血人群血漿sTfR水平高于鐵營(yíng)養狀況正常人群。

關(guān)鍵詞：育齡期婦女；缺鐵性貧血；轉鐵蛋白受體；ROC曲線(xiàn)；臨界值

中圖分類(lèi)號： R153.1 Q581 R556.3 文獻標識碼：A

Study on the critical value of soluble transferring receptor assessing iron deficiency anemia in Chinese child-bearing women

Li Jian-hong, Yang Li-chen, Piao Jian-hua, Yang Xiao-guang

National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention，Beijing 100050, China

Abstract : Objective To compare soluble transferring receptor (sTfR) between iron deficiency anemia and normal iron status in Chinese child-bearing women and study the critical value of sTfR using to assess iron deficiency anemia. Method：251 normal iron status child-bearing women and 224 iron deficiency anemia women were drawn from National Nutrition and Health Survey，2002 as control group and case group respectively. To comprise the sTfR concentration between two groups and ascertain the sTfR critical value to assess iron deficiency anemia by ROC curve. Results：The concentrations of sTfR were （42.67±20.29）nmol/L，（21.08±6.70）nmol/L in case group and control group respectively and the former was higher

than the latter significantly (P<0.01). The optimal critical value of sTfR to assess iron deficiency

anemia was 25nmol/L, corresponding sensitivity and specificity were 87.48%, 80.50% respectively. Conclusion：The sTfR concentration was higher in iron deficiency anemia group than in normal iron status group. The critical value of sTfR diagnosing iron deficiency anemia was sTfR>25nmol/L.

Key words: child-bearing women, iron deficiency anemia, sTfR; ROC curve, critical value

論著(zhù)

文章編號：1000-8020（2007）06-0713-04

一株從酸奶中分離的德氏乳桿菌保加利亞亞種的鑒定和系統發(fā)育分析

汪川張朝武裴曉方劉衡川余倩許欣

四川大學(xué)華西公共衛生學(xué)院醫檢教研室，成都 610041

摘要：目的 對一株分離自酸奶經(jīng)表型特征鑒定為德氏乳桿菌保加利亞亞種的菌株（wch9901）進(jìn)行16S rDNA鑒定和系統發(fā)育分析，為其進(jìn)一步應用和研究奠定基礎。方法提取wch9901基因組DNA作為模板，以16S rDNA兩端的保守序列作為PCR引物，擴增菌株的16S rDNA序列。在T4連接酶的作用下，將擴增得到的16S rDNA序列插入克隆載體pGEM-T，構建重組質(zhì)粒pGEM-wch9901 16S rDNA。重組質(zhì)粒酶切鑒定合格后，交由測序公司對插入的16S rDNA序列進(jìn)行測序。測序結果于GenBank中Blastn，并用軟件Mega3.1以距離依靠法中的鄰位相連法構建系統發(fā)育樹(shù)。結果 wch9901 16S rDNA 序列與Lactobacillus delbrueckii subsp. bulgaricus 中所有菌株的16S rDNA 序列同源性均達96%以上；在系統發(fā)育樹(shù)上wch9901單獨形成一個(gè)分枝，位于Lactobacillus delbrueckii subsp. bulgaricus LGM2遺傳分枝和Lactobacillus delbrueckii subsp. bulgaricus DL2所在的獨立小遺傳簇及Lactobacillus delbrueckii subsp. bulgaricus JSQ所在的獨立小遺傳簇構成的分枝之間，并與Lactobacillus delbrueckii subsp. bulgaricus LGM2遺傳分枝距離最近。結論 wch9901屬于Lactobacillus delbrueckii subsp. bulgaricus；wch9901 與Lactobacillus delbrueckii subsp. bulgaricus LGM2具有最近的親緣關(guān)系。

關(guān)鍵詞：德氏乳桿菌保加利亞亞種 16S rDNA 系統發(fā)育樹(shù)

中圖分類(lèi)號：Q939.117 TS201.3 文獻標識碼:A

Identification and phylogenetic analysis of one strain of Lactobacillus delbrueckii subsp. bulgaricus separated from yoghourt

WANG Chuan, ZHAO Chaowu, PEI Xiaofang, LIU Hengchuan, et al

Department of Medical Technology , West china School of Public Health ,Sichuan University, Chengdu 610041, China

Abstract: Objective For being further applied and studied, one strain of Lactobacillus delbrueckii subsp. bulgaricus （wch9901）separated from yoghourt which had been identified by phenotype characteristic analysis was identified by 16S rDNA and phylogenetic analyzed. Methods The 16S rDNA of wch9901 was amplified with the genomic DNA of wch9901 as template, and the conservative sequences of the 16S rDNA as primers. Inserted 16S rDNA amplified into clonal vector pGEM-T under the function of T₄ DNA ligase to construct recombined plasmid pGEM-wch9901 16S rDNA. The recombined plasmid was identified by restriction enzyme digestion, and the eligible plasmid was presented to sequencing company for DNA sequencing. Nucleic acid sequence was blastn in GenBank and phylogenetic tree was constructed using neighbor-joining method of distance methods by Mega3.1 soft. Results Results of blastn showed that the homology of 16S rDNA of wch9901 with the 16S rDNA of Lactobacillus delbrueckii subsp. bulgaricus strains was higher than 96%. On the phylogenetic tree, wch9901 formed a separate branch and located between Lactobacillus delbrueckii subsp. bulgaricus LGM2 evolution branch and another evolution branch which was composed of Lactobacillus delbrueckii subsp. bulgaricus DL2 evolution cluster and Lactobacillus delbrueckii subsp. bulgaricus JSQ evolution cluster. The distance between wch9901 evolution branch and Lactobacillus delbrueckii subsp. bulgaricus LGM2 evolution branch was the closest. Conclusion Wch9901 belonged to Lactobacillus delbrueckii subsp. Bulgaricus; wch9901 showed the closest evolution relationship to Lactobacillus delbrueckii subsp. bulgaricus LGM2.

Key words: Lactobacillus delbrueckii subsp. Bulgaricus, 16S rDNA, phylogenetic tree

作者簡(jiǎn)介：汪川，女，講師，博士

論著(zhù)

文章編號：1000-8020（2007）06-調查研究

林州市食管癌高發(fā)區人群維生素營(yíng)養狀況的動(dòng)態(tài)變化研究

呂全軍胡同宇朱明君王旗陸維權1 薛樂(lè )勛2

劉志才3連士勇3李變云3程蘭平3魏建榮3

鄭州大學(xué)公共衛生學(xué)院，鄭州 450052

摘要： 目的了解林州市食管癌高發(fā)區人群維生素營(yíng)養狀況的動(dòng)態(tài)變化。方法采用生態(tài)學(xué)對比研究的方法，在林州市選擇曾經(jīng)進(jìn)行過(guò)營(yíng)養調查的8個(gè)鄉,每鄉2村,每村隨機抽取30戶(hù)居民為研究對象，采集血樣和4h負荷尿，測定血清維生素A，血漿總抗壞血酸，紅細胞谷胱甘肽還原酶活性系數分別采用三氟乙酸比色法，2，4二硝基苯肼法和Sauberlich法，尿中核黃素，還原型抗壞血酸，總抗壞血酸排出量分別采用熒光法，2，6二氯酚靛酚滴定法和2，4二硝基苯肼法，測定結果與1982年，1983年或1984年的調查結果進(jìn)行比較分析，采用采用t檢驗或c2檢驗。結果任村、東姚和河澗三個(gè)鄉的居民血清維生素A顯著(zhù)高于1982年的結果（P <0.01）；姚村、任村和河順三個(gè)鄉的居民4h負荷尿核黃素排出量顯著(zhù)高于1983年或1984年的結果（P <0.01）；以4h負荷尿核黃素排出量和紅細胞谷胱甘肽還原酶活性系數評價(jià)核黃素營(yíng)養狀況發(fā)現東姚、河澗、姚村、任村四個(gè)鄉的居民核黃素缺乏的人數顯著(zhù)低于1982年或1983年的結果（P<0.01）；以血漿總抗壞血酸水平及4h負荷尿中抗壞血酸排出量平均值評價(jià)維生素C營(yíng)養狀況發(fā)現東姚、河澗、姚村、任村和河順五個(gè)鄉的居民總抗壞血酸缺乏的人數顯著(zhù)低于1982年或1983年的結果（P<0.01）。結論食管癌高發(fā)區林州市居民維生素A，B2，C的營(yíng)養狀況有了明顯的改善。

關(guān)鍵詞：食管癌維生素營(yíng)養狀況

中圖分類(lèi)號： R735.1 R151.42 Q56 文獻標識碼：A

study on changes of vitamin nutrition status of population in a high-risk area of esophageal cancer

Lu Quanjun , Wang Huali , Hu Tongyu，et al

College of Public Health, Zhengzhou University, Zhengzhou 450052 , China

Abstract: Objective To know the changes of vitamin nutritional status of residents in a high-risk area of esophageal cancer. Methods 8 towns where nutrition survey had been done were selected, 2 villages were randomly selected from each town and 30 families were randomly selected per village. The blood and 4h burden urine samples were collected from subjects. The serum vitamin A, blood total ascorbic acid and glutathione reductase activition coefficient(AC) in red blood cell were determinde by using of trifluoroacetic acid colorimetry， 2,4-dinitrophenylhydrazine colorimetric method and Sauberlich method respectively; The contents of vitamin B2 , reduction ascorbic acid and total ascorbic acid in 4h burden urine were measured by using of fluorescent pectrophotometry,2,6-Dichloro-N-(4-hydroxyphenyl)-1,4-benzoquinoneimine titrimetric method and 2,4-dinitrophenylhydrazine colorimetric method respectively. The results were analyzed and compared with those of in 1980, 1983 and 1984.The t-test or c2-test was carried out. Results The level of serum retinal in residents living in Ren cun,Dongyao and Hejian is significantly higher than that of in 1982（P <0.01）；The level of vitamin B2 in 4h burden urine in residents living in Yao cun, Ren cun and Heshun are significantly higher than that of in 1982 or 1984（P <0.01）；the number of ribflavin deficincy of population living in Dongyao, Hejian,Yao cun and Ren cun are significantly lower than that of in 1982 or 1983（P <0.01）according to the content of 4h burden urine and glutathione reductase activition coefficient(AC) in red blood cell; the number of vitamin C deficincy of population living in Dongyao, Hejian,Yao cun ,Ren cun and Heshun are significantly lower than that of in 1982 or 1983（P <0.01）according to blood total ascorbic acid and the ascorbic acid content of 4h-burden urine and. Conclusion vitamin nutritional status of residents in a high-risk area of esophageal cancer have been improved.

Key words: esophageal cancer, vitamin, nutrition status

基金項目：教育部“211工程”項目

作者簡(jiǎn)介：呂全軍，男，博士，副教授，E-mail:lqjnutr@zzu.edu.cn

1.河南省腫瘤研究所

2.通訊作者：薛樂(lè )勛,鄭州大學(xué)細胞生物學(xué)研究室,E-mail: xuelx@371.net

3.河南省林州市食管癌防治研究所腫瘤醫院

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青年科學(xué)工作者論壇2007年第6期