青年科學(xué)工作者論壇2007年第5期
論著(zhù)
文章編號：1000-8020（2007）05-0555-04
綠茶多酚對心理應激大鼠行為學(xué)表現的干預作用研究
陳偉強 程義勇 趙小玲 李樹(shù)田 侯玥 洪燕
軍事醫學(xué)科學(xué)院衛生學(xué)環(huán)境醫學(xué)研究所，天津 300050
摘要：目的 觀(guān)察綠茶多酚對心理應激大鼠自主探究行為、
關(guān)鍵詞：心理應激 綠茶多酚 行為表現 皮質(zhì)醇
中圖分類(lèi)號：R151.2 R338.6 TS272.51 文獻標識碼：A
Study of green tea polyphenols modulation on behavioral performances in psychological stress rats
CHEN Weiqiang, CHENG Yiyong, ZHAO Xiaoling, LI Shutian, et al.
Institute of Health and Environmental Medicine, Tianjin 300050, China
Abstract：Objective This study was conducted to explore the effects of green tea polyphenols modulation on changes of behavioral performances in psychological stress rats. Methods The animal model of psychological stress was developed by restraint stress for 3 weeks. Wistar rats were randomly assigned to five groups as follows: control group (CT), stress control group (SCT), and three stress groups with low, medium and high-doses of green tea polyphenols modulation respectively (SLG, SMG and SHG). The changes of behavioral performances were examined by open-field test, water maze and step-through test. Serum levels of cortisol, catecholamines, interleukin-6 and interleukin-2 were also detected. Results The levels of serum cortisol were all increased obviously in the four stress groups. Furthermore, serum cortisol levels in SMG and SHG were decreased than that of SCT. The behavioral performances of SCT rats in open-field test, step-through test and water maze were all changed evidently in contrast to that of CT rats. On the one hand, the changes of behavioral performances in SLG rats were similar to SCT rats. On the other hand, these changes were improved in SMG and SHG rats. In addition, compared with CT group, the levels of plasma IL-6 and IL-1 were increased clearly in the four stress groups, and the contents of serum norepinephrine and dopamine in SCT and SLG groups were decreased dramatically. The serum norepinephrine and dopamine levels in SMG and SHG rats were increased in contrast to that of SCT rats. Conclusion Our results suggested that psychological stress can impair body’s behavioral performances, and moderate green tea polyphenols modulation may improve these abnormal changes.
Key words: psychological stress, green tea polyphenols, behavioral performances, cortisol
基金項目：國家自然科學(xué)基金資助項目（No.30500406）
作者簡(jiǎn)介：陳偉強，男，醫學(xué)博士，副研究員，E-mail：ch
論著(zhù)
文章編號：1000-8020（2007）05- 0542-03
中國60歲及以上人群空腹血糖分布及糖尿病流行特征
付萍 滿(mǎn)青青 張堅 王春榮 楊曉光
中國疾病預防控制中心營(yíng)養與食品安全所，北京 100050
摘要：目的 使用中國居民營(yíng)養與健康狀況調查（2002年）的數據，分析60
關(guān)鍵詞 ：老年人 空腹血糖 高血糖狀態(tài) 糖尿病
中圖分類(lèi)號：R181.37 R587.1 R153.3 文獻標識碼：A
Epidemiological study on diabetes mellitus in people aged 60 and over
FU Ping, MAN Qingqing, ZHANG Jian, WANG Chunrong,et al.
Institute of Nutrition and Food Satety, China CDC, Beijing 100050, China.
Abstract：Objective To study the epidemiological characteristics of diabetes mellitus in Chinese people aged 60 and over based on the data of China Nutrition and Health Survey in 2002. Methods Fasting plasma glucose (FPG) of 9925 subjects, aged 60 and over was determined by glucose oxidase method and the prevalence of diabetes mellitus was analyzed by gender and by regions. Results The average FPG of all subjects was 5.18mmol/L adjusted by population distribution based on 2000 census. Over 29.5% of subjects in metropolis and 21.6% middle cities were determined as fasting hyperglycemia (FPG≥5.6mmol/L). The prevalence of DM in old subjects was 6.83%。The prevalence of DM in old subjects of metropolis, middle city, 1st, 2nd ,3rd , 4th country was 16.97%，11.43%，4.94%，4.70% and 1.94% respectively. Conclusion Over 21% of Chinese urban residences aged 60 and over suffer from fasting hyperglycemia and the prevalence of DM reached 13.17%..
Key words: old people, fast plasma glucose, hyperglycemia, diabetes mellitus prevalence.
論著(zhù)
文章編號：1000-8020（2007）05-0547-05
學(xué)齡兒童體內植物源性胡蘿卜素轉化成維生素A的效率研究
李蕾 王茵1 武潔姝 朱染楓1 趙顯峰 汪之頊2 蔭士安3
中國疾病預防控制中心營(yíng)養與食品安全所，北京 100050
摘要：目的 確定菠菜及純品油膠囊中β-胡蘿卜素在學(xué)齡兒童體內的轉化效率。
關(guān)鍵詞：β-胡蘿卜素 維生素A 生物轉化 學(xué)齡兒童
中圖分類(lèi)號： R153.2 Q562 Q58 文獻標識碼：A
Bioefficacy of plant carotenoid in school age children
LI Lei ,WANG Yin .WU Jieshu,ZHU Ranfeng,et al.
Institute of Nutrition and Food Safety, Chinese Centre for Disease Control and Prevention, Beijing 100050, China
Abstract：Objective To determine the bioefficacy of pure β-carotene and β-carotene from spinach in schoolchildren of different vitamin A status. Methods: Thirty two schoolchildren aged 7-9 years old were selected and then divided into two groups randomly. Each group included 16 subjects, of which the vitamin A level of 8 subjects was normal, the other 8 subjects suffered from vitamin A deficiency. In the first 7 days, one group was given 5 gram spinach (containing 230μg 2H10-β-carotene) before lunch and dinner and the other group was given pure β-carotene capsule (containing 200μg β-carotene). Before breakfast, both groups were given 100μg 13C10 retinal acetate capsule. Blood was drawn on the 3,7,8,22 and 28 day. HPLC and GC-MS were used to determine the retinol concentration and enrichment of isotope and then calculate the bioefficacy of pure β-carotene and β-carotene from spinach. Results: The conversion factors of pure β-carotene in oil capsule were: 2.9 for the children with normal vitamin A status, 3.2 for those suffering from marginal vitamin A deficiency. The conversion factors of spinach β-carotene were 10.1and 10.3 for the children with normal and low vitamin A status, respectively. Conclusion: The vitamin A conversion bioefficacy of pure β-carotene from oil capsule is much higher than that of spinach. There is no difference of β-carotene bioefficacy for children with different vitamin A status.
Key words：β-carotene, vitamin A, bioconversion, school-aged children
基金項目：國家自然科學(xué)基金資助項目（No.30571574）
作者簡(jiǎn)介：李蕾，女，博士生
1浙江省醫學(xué)科學(xué)院保健食品研究所
2青島大學(xué)醫學(xué)院營(yíng)養研究所
3通訊作者