楊年紅 王重建 許明佳 胡學(xué)鋒 郝麗萍 劉烈剛 孫秀發(fā)
華中科技大學(xué)同濟醫學(xué)院公共衛生學(xué)院營(yíng)養與食品衛生系，武漢 430030

摘要：
　　目的 探討白色脂肪組織UCPs和PPARγ2基因在高脂飲食誘導大鼠肥胖抵抗中的作用。
　　方法 36只雌性SD大鼠，按體重隨機分為高脂實(shí)驗組和基礎對照組，分別給予高脂飼料和基礎飼料13周。實(shí)驗結束時(shí)，根據體重將高脂組大鼠分為飲食誘導肥胖(DIO)和飲食誘導肥胖抵抗 (DIO-R)大鼠，比較各組大鼠體重、脂肪濕重、脂體比、空腹血糖(FBG)及血脂譜；RT-PCR法測定白色脂肪組織UCP2、UCP3及PPARγ2 mRNA的表達水平。
　　結果 DIO-R及DIO大鼠脂體比、TC、LDL-C、TG等指標均顯著(zhù)高于對照組相，但DIO-R組體重、脂肪濕重、脂體比、TG顯著(zhù)低于DIO組(P<0.05)； DIO-R大鼠UCP2和UCP3 mRNA的表達水平高于DIO大鼠和對照組(P<0.01)，PPARγ2 mRNA的表達水平低于DIO大鼠(P<0.01)，高于對照組(P<0.01)。結論 高脂飲食誘導大鼠發(fā)生肥胖抵抗與白色脂肪組UCP2、UCP3高表達及PPARγ2 mRNA表達下降密切相關(guān)。

關(guān)鍵詞：高脂飲食 肥胖抵抗 解偶聯(lián)蛋白 過(guò)氧化物酶體增值物激活受體γ2
中圖分類(lèi)號：R1511 文獻標識碼：A

UCPs and PPARγ2 mRNA in diet induced obesity resistant rats

Yang Nian-hong, Wang Chong-jian, Xu Ming-jia, Hu Xue-feng, et al.

Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College,Huazhong University of Science and Technology, Wuhan 430030, China

Abstract：Objective To explore the expression of uncoupling proteins (UCP) and peroxisome proliferator activated receptorγ2 (PPARγ2) mRNA in white adipose of highfat diet induced obesity resistant rats. Methods Thirtysix female SD rats were randomly divided into highfat diet group (n=27) and chow fed control group (n=9), and given either highfat diet or chow for thirteen weeks. Then the highfat diet group was subdivided into Dietary Induced Obesity (DIO) and Dietary Induced Obesity Resistant (DIOR) group according to the final body weight. Basic parameters, fasting blood glucose (FBG), blood lipid levels were measured and compared. Reverse transcriptionpolymerase chain reaction (RT-PCR) was used to measure the expression of UCP2, UCP3, and PPARγ2 mRNA in white adipose. Results Adipose deposits, body fat percent, TC, and TG conceration of both DIO-R and DIO groups were significantly higher than those in control group, while body weight，body fat percent and TG level were significantly lower in DIO-R than DIO rats. The expression of UCP2 and UCP3 mRNA in white adipose of DIO-R rats were significantly higher than those of DIO and control group. The expression of PPARγ2 mRNA in white adipose of DIO-R group was significantly lower than that in DIO group. Conclusion Highfat diet induced obesity resistance were closely associated with the increased UCP2, UCP3, and decreased PPARγ2 mRNA in white adipose.

Key words:highfat diet， obesity resistance， UCP2, UCP3, PPARγ2

基金項目：國家自然科學(xué)基金資助項目（ No.30371213 ）；湖北省衛生廳基金資助項目（ No.LJ200212 ）
作者簡(jiǎn)介：楊年紅，女，副教授，博士研究生

李珂 姚平 周紹良 宋方方 章錫平 孫秀發(fā) 劉烈剛1
華中科技大學(xué)同濟醫學(xué)院公共衛生學(xué)院營(yíng)養與食品衛生學(xué)系,武漢 430030

摘要：
　　目的 研究銀杏葉提取物(EGB)對酒精所致大鼠睪丸氧化損傷的預防保護作用。
　　方法 雄性SD大鼠30％酒精灌胃(2.37g/kg bw)前，EGB采用分組(低劑量組48μg/g bw和高劑量組96μg/g bw)預防性給藥的方法。于實(shí)驗90d檢測大鼠睪丸組織中超氧化物歧化酶(SOD)、谷胱甘肽轉移酶(GST)、谷胱甘肽過(guò)氧化物酶(GSH-Px)、過(guò)氧化氫酶(CAT)、和谷胱甘肽(GSH)、活性氧(ROS)以及丙二醛(MDA)的含量。通過(guò)亞細胞分離方法提取大鼠睪丸微粒體，測定微粒體血紅素氧化酶-1(HO-1)活性。采用RT-PCR檢測睪丸組織中血紅素氧化酶-1(HO-1)mRNA表達水平。
　　結果 慢性酒精攝入90d后，EGB組睪丸勻漿ROS、MDA較酒精組有明顯降低(P<0.05)；相反， GST、G-Px、CAT、SOD、HO-1活性以及GSH均有顯著(zhù)性升高(P<0.05)；EGB誘導HO-1高表達。結論 長(cháng)期慢性酒精攝入引起大鼠睪丸氧化損傷。EGB可作為一種預防性的抗氧化劑減輕這種氧化損傷；其機制可能與誘導HO1高表達，清除自由基，抑制脂質(zhì)過(guò)氧化反應有關(guān)。

關(guān)鍵詞：銀杏葉提取物 氧化損傷 抗氧化酶 血紅素氧化酶-1
中圖分類(lèi)號：R961〓Q579.1 文獻標識碼：A

Protective effects of extract of ginkgo biloba againstethanol  induced oxidative injury in rat testes

Li Ke, Yao Ping, Zhou Shao-liang, Song Fang-fang, et al.

Department of Nutrition and Food Hygiene, School of Public Health,Tongji MedicalCollege, HuazhongUniversity of Science and Technology, Wuhan 〓 430030, China

Abstract ： Objective This study was designed to investigate whether EGB protect against ethanol induced oxidative injury in rat testes. Methods The rats were pretreated with EGB (4 . 8 ， 9 . 6mg/ 100g bw per day) before 30 ％ ethanol administration ( 2 . 37g /kg bw by gastric incubation). Ninety days later, the rats were killed in order to measure the activities of Superoxide Dismutase(SOD), Glutathione Reductase(GST), Glutathione Peroxidase(GSH - Px), Catalase(CAT) and the contents of Glutathione(GSH),Reactive Oxygen Species(ROS), Malondialdehyde(MDA)in rat testes. The microsomal fractions were obtained by the method of differential centrifugation, and HO - 1(Heme Oxygenase - 1) activity in testicular microsomal was measured. To examine the expression of HO - 1 mRNA by reverse transcription polymerase chain reaction (RT - PCR). Results Compared with ethanol group, ROS, and MDA contents significantly decreased in EGB group testes, respectively(P<0 . 05). GST, GSH  Px, CAT, SOD activities, and GSH content in EGB group testes elevated markedly(P<0 . 05).EGB could enhance HO - 1mRNA expression remarkably. Conclusion It is suggested that EGB as a preventive antioxidant can protect against ethanol  induced testicular injury ， which may be associated with HO - 1 activity enhancement, free radicals elimination, lipid per  oxidation reaction inhibition.

Key words: extracts of Ginkgo biloba(EGB), oxidative injury, antioxidant enzyme, heme oxy Genase - 1(HO - 1)

基金項目：國家自然科學(xué)基金項目 (No.30271130)
作者簡(jiǎn)介：李珂，女，碩士研究生
1 通訊作者

蘇麗琴 綜述 金銀龍 審校
中國疾病預防控制中心環(huán)境與健康相關(guān)產(chǎn)品安全所，北京 100021

摘要： 慢性砷中毒對人體健康的損害嚴重而且危害廣泛，一直是世界各國廣泛關(guān)注的問(wèn)題。本文首先簡(jiǎn)要介紹了慢性砷中毒的類(lèi)型和主要臨床表現，然后從砷的接觸史、臨床表現和實(shí)驗室檢查三個(gè)方面介紹國內外大規模現場(chǎng)人群調查的研究現狀，并討論這三方面研究對慢性砷中毒判定的意義，最后提出了進(jìn)一步開(kāi)展慢性砷中毒人群流行病學(xué)調查的幾點(diǎn)思考。

關(guān)鍵詞： 慢性暴露 砷中毒 流行病學(xué) 判定
中圖分類(lèi)號： R181 . 23 R599 . 9 R994 . 6 文獻標識碼： A

Significance of field epidemiologic study to identificationof chronic arsenic poisoning

Su Li - qin, Jin Yin - long

Institute for Environmental Health and Related Product Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China

Abstract ： Chronic arsenic poisoning has serious and extensive impact on human health, which attracts wide attention worldwide. Bases on vast public survey, this article introduces recent field studies on chronic arsenic poisoning from three aspects: exposure history, clinical symptoms and laboratory evidences, and also explains the meaning of each index to the determination of chronic arsenic poisoning, then bring forward some considerations on further epidemiological studies on chronic arsenic poisoning.

Key words: chronic exposure, arsenic poisoning, epidemiology, determination

基金項目 ：環(huán)境污染對人體健康的影響與損害診斷技術(shù)研究
作者簡(jiǎn)介：蘇麗琴，女，碩士研究生