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青年科學(xué)工作者論壇2005年第6期

黃河三角洲水源性高碘地區分布與居民食用鹽現狀調查研究

郭曉尉 翟麗屏 劉源 王欣 劉傳蛟 秦啟亮 陳祖培 1
山東省地方病防治研究所,濟南 250014


摘要:
  目的 查清黃河三角洲的水源性高碘地區分布和居民食用鹽現狀以制定干預策略。
  方法 采用橫斷面調查方法,每個(gè)被調查縣的所有鄉鎮均按東、西、南、北、中五個(gè)不同方位抽取5個(gè)村,在每個(gè)村采集飲用水樣2份進(jìn)行水碘含量檢測,并了解飲水源類(lèi)型及水井深度。每個(gè)村隨機采集5個(gè)戶(hù)居民食用鹽進(jìn)行定性檢測。結果 調查8個(gè)縣92個(gè)鄉鎮的451個(gè)村莊,檢測水樣800份、食鹽800份。水碘含量均值為(110.93±152.26)μg/L,中位數為55.83μg/L(0.84~997.82μg/L);96.5%為平原地區水源。井深平均(70.44±136.19)m,中位數12.0m(2.0~999.0m)。水碘>150μg/L的高碘地區,分布于7個(gè)縣的24個(gè)鄉鎮,受累人口102.39萬(wàn)人,碘鹽覆蓋率97.2%;其水碘含量為(327.72±192.19)μg/L,中位數253.87μg/L(150.78~997.82μg/L)。淺層井水的高碘含量可能與黃河泛濫把從上游攜帶來(lái)的富碘泥沙的長(cháng)期沉積有關(guān),而深井水高碘成因除此之外還與三角洲的沉積及海底大陸架千萬(wàn)年來(lái)沉積的富碘海洋生物有關(guān)。
  結論 黃河三角洲有24個(gè)高碘地區,碘鹽覆蓋率97.2%,既有淺井高碘水又有深井高碘水的獨特現象。高碘地區應停止碘鹽供應措施,開(kāi)展健康教育和監測工作。

關(guān)鍵詞:水碘 高碘地區 碘鹽 黃河三角洲
中圖分類(lèi)號:R151.42 R123.1 R599.9 文獻標識碼:A

Study on the present status of the areas with high iodine concentration indrinking water and edible salt at household levels in Ohio of Yellow River

Guo Xiao - wei, Zhai Li - ping, Liu Yuan, Wang Xin,et al.

Shandong Institute for Endemic Disease Control and Research, Jinan 250014, China

Abstract : Objective To understand the present condition of iodine excess areas and edible salt at household levels in Ohio of Yellow River , which will provide the evidence to control it. Methods A cross section in one time was adopted for the epidemiological survey based on the east, west, south, north and central in all of townships from 8 counties. 2 samples of drinking water from each village were tested their water iodine content as well as the data regarding to their recourses and the depth of wells. 5 samples of edible salt were collected from each village for quantitative analysis. Results We investigated 451 villages in 92 townships of 8 counties. 800 samples of drinking water were tested which values of iodine content were (110 . 93 ± 152 . 26) μ g/L in main, 55 . 83 μ g/L(0 . 84 , 997 . 82 μ g/L)in medium. 102 . 39 thousand population are at risk for iodine excess and living in 24 townships of 7 counties where iodine concentration is over 150 μ g/L in drinking water, with (327 . 72 ± 192 . 19) μ g/L in mean value or 253 . 87 μ g/L(150 . 78 , 997 . 82 μ g/L)in medium. The rate of iodized salt is 97 . 2%. All the iodine excess areas are located in alluvial plain of Yellow River. The etiology of high iodine in shallow well water may be supposed to be iodine aggregation formed by Yellow River in terms of thousands of flood in thousands of years. But iodine excess in deep well water may be related to rotten, deposit marine living beings rich in iodine millions upon millions years ago. Conclusion There were distinctive features of iodine excess in drinking water from both shallow well and deep well, 24 iodine excess areas in Ohio of Yellow River. It has suggested that iodized salt intervention should be stopped in the areas and starting the health education project, survey of iodized salt in the region.

Key words: water iodine, iodine excess areas, iodized salt, Ohio of Yellow River

基金項目:國家自然科學(xué)基金重點(diǎn)項目( No. 30230330 );國際控制碘缺乏病理事會(huì )( ICCIDD );衛生部疾控司資助項目( 2003 )
作者簡(jiǎn)介:郭曉尉,男,副主 任 醫師
1 天津醫科大學(xué)內分泌研究所


 

沙門(mén)菌、志賀菌和大腸桿菌 O157 ∶ H7 的多重 PCR 快速檢測體系的初步探討
2006年8月17日

何超 樊學(xué)軍1 汪東籬 劉麗英2 王翠蘋(píng)2 裴曉方3
四川大學(xué)華西公共衛生學(xué)院,成都 610041


摘要:
  目的 建立能在12小時(shí)內同時(shí)快速檢測沙門(mén)菌、志賀菌和大腸桿菌O157∶H7的多重PCR體系。
  方法 堿性蛋白胨水(BPW)非選擇性增菌6h;100℃10min制備DNA模板;根據大腸桿菌O157∶H7的uidA基因、志賀菌的ipaH基因及沙門(mén)菌的invA基因序列設計各菌引物,進(jìn)行PCR擴增及電泳檢測。同時(shí)優(yōu)化反應體系,測定體系靈敏度和特異性。
結果 該多重PCR體系能在12h內同時(shí)檢測3種目的菌;靈敏度為10~30cfu/ml;通過(guò)對23株目的菌和15株非目的菌檢測,提示該體系特異性高。
  結論 初步探討出能在12h內快速、靈敏、特異地同時(shí)測定沙門(mén)菌、志賀菌和大腸桿菌O157∶H7的多重PCR體系。

關(guān)鍵詞:多重PCR 沙門(mén)菌 志賀菌 大腸桿菌O157∶H7
中圖分類(lèi)號:R378.2 R155.31 文獻標識碼:A

Primarily study on a multiplex PCR based system for the rapid detection of Salmonella spp., Shigella spp. and Escherichia coli O157 H7

He Chao, Fan Xue - jun, Wang Dong - li, Liu Li - ying, et al.

West China School of Public Health, Sichuan University , Chengdu 610041, China

Abstract:Objective To establish a multiplex PCRbased system for the simultaneous detection of Salmonella spp., Shigella spp. and Escherichia coli O157∶H7 in 12 hours.Methods After 6 h nonselective enrichment in BPW, DNA template were prepared at 100℃ for 10min. Three sets of primers were designed to amplify the gene segments of invA of Salmonella spp, ipaH of Shigella spp, and uidA of E. coli O157∶H7, and the products were analyzed by electrophoresis. At the same time, this system was optimized,and the specificity and sensitivity of this system were evaluated.Results Three target bacteria were detected in 12 h by using this multiplex PCRbased system. The sensitivity of it was up to 10-30cfu/ml, and the high specificity was demonstrated by detecting 23 target stains and 15 nontarget stains.Conclusion A rapid,specific, and sensitive multiplex PCRbased system for the simultaneous detection of Salmonella spp., Shigella spp. and E. coli O157∶H7 in 12 h has been studied primarily.

Key words:multiplex PCR, Salmonella spp., Shigella spp, Escherichia coli O157∶H7

基金項目:國家質(zhì)檢總局科研計劃項目( No.J2005J0115 )
作者簡(jiǎn)介:何超,女,碩士研究生
1 四川省檢驗檢疫局國際旅行衛生保健中心
2 四川大學(xué)華西公共衛生學(xué)院 99 級衛檢本科生
3 聯(lián)系作者

 
不同體脂人群腸道主要菌群的定量分析

劉祥 張朝武 潘素華 裴曉方 余倩1
四川大學(xué)華西公共衛生學(xué)院,成都 610041

摘要:
  目的 探討主要腸道菌群在不同體脂人群間數量變化及其對脂質(zhì)代謝可能具有的影響。
  方法 照BMI分類(lèi)標準,將研究對象分為消瘦組、正常組、超重組、肥胖組,采用選擇性培養法測定研究對象新鮮糞便中5種主要腸道菌群的數量。結果 隨著(zhù)BMI值的升高,乳酸桿菌、雙歧桿菌和腸桿菌數量呈下降趨勢,擬桿菌數量則顯著(zhù)升高(P<0.01),腸球菌和產(chǎn)氣莢膜梭菌的數量無(wú)明顯的變化趨勢。
  結論 擬桿菌對脂肪積累和肥胖形成可能具有一定的促進(jìn)作用,而腸桿菌、乳酸桿菌和雙歧桿菌則可能起相反的作用。

關(guān)鍵詞:肥胖 BMI 腸道菌群 微生態(tài)
中圖分類(lèi)號:R378.2 R589.2 文獻標識碼:A

Quantitative analysis of dominating intestinal flora amonggroups of people with different body fat

Liu Xiang, Zhang Chao - wu, Pan Su - hua, Pei Xiao - fang, et al.

West China School of Public Health, Sichuan University , Chengdu 610041, China

Abstract : Objective To investigate the alteration of dominating intestinal floras among groups of people with different body fat and probe into the possible effect on lipid metabolism. Methods According to the BMI values, subjects were divided into 4 groups, including fleshless group, normal group, overweight group and obese group. Five dominating floras of all fresh stools were quantitated using selective culture method, and all data were analyzed statistically. Results With the increase of BMI values, there is a decreasing trend in the amount of  Lactobacillus, Bifidobacterium  and  Enterobacillus  , and the amounts of  Bacteriodes  obviously increased (P<0 . 01). No obvious alternation of the amounts of  Enterococcus  and  Clostridium  was observed. Conclusion Bacteriodes may exert an boosting effect on the pile of fat and development of obese, however Enterobacillus, Lactobacillus, and Bifidobacterium have a potential opposite effect.

Key words: obesity , BMI , intestinal flora , microecology

作者簡(jiǎn)介:劉祥,男,博士研究生
1:通訊作者

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