文章編號：1000-8020（2006）04-0426-02

 

沈慧  秦海宏  龍建綱  王福俤  郭俊生

第二軍醫大學(xué)軍隊衛生學(xué)教研室，上海  200433

 

摘要：目的  研究鋅對Caco2細胞ZIP4 mRNA表達的影響及其規律。方法  通過(guò)鋅特異螯合劑TPEN建立低鋅Caco2細胞模型，RT-PCR法獲得ZIP4 cDNA片斷，10μM TPEN培養基誘導后，分別檢測0、2、4、6、8和10h時(shí)點(diǎn)ZIP4 mRNA的表達，及0、2.5、5、7.5、10μmol/L TPEN培養基誘導6h，檢測各濃度組ZIP4 mRNA的表達。結果  RT-PCR獲得單一條帶的片斷，大小與設計一致，獲得正確的ZIP4 cDNA片斷，隨著(zhù)低鋅時(shí)間的增加，ZIP4 mRNA表達也升高，6h達到峰值；隨著(zhù)TPEN濃度的升高，ZIP4 mRNA表達也隨之升高。結論  ZIP4 mRNA表達受鋅的調控，提示可能參與小腸對鋅的吸收。

關(guān)鍵詞： 鋅  鋅轉運體  鋅鐵調控蛋白

 

 

Abstract: Objective  To study the effect of zinc on mRNA expression of ZIP4 in human intestinal Caco2 cells and its regularity. Methods  Low zinc cell model was established by TPEN, a kind of chelating agent which chelates specially to zinc. ZIP4 cDNA fragment was obtained by RT-PCR. Expression of ZIP4 on 10μM TPEN exposure after 0, 2, 4, 6, 8 and 10 hours in Caco2 cells and its expression on various concentration of TPEN exposure (0,2.5,5,7.5 and 10μmol/L) was measured by RT-PCR. Results  A proper single fragment is obtained with the sequence conformable to the design. A proper single ZIP4 cDNA fragment was obtained. The mRNA expression of ZIP4 increased in accordance with the duration of low zinc. The peak mRNA level appeared at about 6h. And the ZIP4 mRNA increased in accordance with the concentration of TPEN in Caco2 cells. Conclusion  Zinc can regulate the mRNA expression of ZIP4 in Caco2 cells. And ZIP4 may play a role in the absorption of zinc in human intestine.

Key words: zinc, zinc transporter, ZRT,IRT-like protein

 

 

基金項目：國家自然科學(xué)基金資助項目（No.30170805）

作者簡(jiǎn)介：沈慧，男，博士，講師

楊莉  楊維東  劉潔生¹  江濤

暨南大學(xué)生命科學(xué)技術(shù)學(xué)院，廣州  510632

 

摘要：目的  對廣州市售雙殼經(jīng)濟貝類(lèi)麻痹性貝毒（PSP）和腹瀉性貝毒（DSP）污染狀況進(jìn)行為期一年的抽樣調查，了解其食用安全性。方法  采用AOAC推薦的小鼠生物檢測法進(jìn)行PSP和DSP的毒力測定，采用HPLC進(jìn)行PSP成分分析，根據FAO、日本和歐盟水產(chǎn)食品衛生要求及我國漁政漁港監督管理局制訂的貝類(lèi)安全食用標準對貝類(lèi)水產(chǎn)品的食用安全性進(jìn)行評價(jià)。結果 在所調查的7種貝類(lèi)中，有2種染有PSP，毒素含量在安全食用范圍內，毒力大小隨季節而變化，春冬兩季含量相對較高；7種貝類(lèi)中有6種共計36個(gè)樣品染有DSP，有10個(gè)樣品毒素含量超出安全食用標準，春冬兩季染毒率較高。結論 廣州市售貝類(lèi)PSP含量和檢出率整體水平較低；DSP檢出率稍高，毒素含量也較高，應引起有關(guān)部門(mén)的關(guān)注，加強DSP監測工作。

關(guān)鍵詞：麻痹性貝毒  腹瀉性貝毒  貝類(lèi)毒素  監測

 

Yang Li, Yang Wei-dong, Liu Jie-sheng, Jiang Tao

College of Life Science and Technology, Jinan University, Guangzhou 510632,China

Abstract：Objective  Paralytic shellfish poisoning (PSP) and diarrhetic shellfish poisoning (DSP) in 7 species of economical shellfishes were analyzed for 1 year, which collected from Huangsha seafood market of Guangzhou from Apr, 2004 to Mar, 2005. Methods  The levels of PSP and DSP in bivalves were determined with mouse bioassay of AOAC. The risk assessment of PSP and DSP in bivalves was conducted according to FAO and Chinese Administration Organization of Fish Culture and Seaport. Results  PSP was detected in 2 species of the shellfishes assayed and DSP was found out in 6 species. The content of PSP was lower than 4MU/g tissue, whereas the level of PSP in glands was higher than in muscles. DSP toxin was detected in 36 samples of 6 species, what is more, DSP level in 10 samples exceeded the safety threshold. The levels in PSP and DSP of bivalves were all higher in spring and winter with some characteristic of season. Conclusin  The results suggested that PSP in economical shellfish in Guangzhou market was lower, and shellfish was safe to eat in term of the PSP level if glands were discarded, but DSP contamination in bivalves was severe. It is essential to detect and assess the risk of DSP and PSP in bivalves from seafood market in the future.

Key words：paralytic shellfish poisoning, diarrhetic shellfish poisoning, toxins, detection

 

 

基金項目：國家重點(diǎn)基礎研究發(fā)展規劃項目“973” (No.2001CB409710，.2001CB409709 )；廣東省科技計劃項目(No.2003C20412)；廣州市科技計劃項目(No.2002J1-C0011)

作者簡(jiǎn)介：楊莉，女，碩士

1通訊作者

　           婁曉民  段廣才¹  陳姜¹  周艷¹  胡巧云¹  衡正昌

四川大學(xué)華西公共衛生學(xué)院，成都610041

 

摘要：目的  分析兒童肥胖形成中肥胖發(fā)生調節因子及與青春期發(fā)育相關(guān)激素的變化，探討這些激素在青春期兒童肥胖形成中的變化特點(diǎn)及其相互關(guān)系。方法 以78例肥胖的13歲兒童和84例同歲正常對照兒童為研究對象，以放射免疫法測定血中食欲素（orexins）、瘦素（leptin）、胰島素（insulin） 及睪酮（T）、雌二醇（E₂）、黃體生成素（LH）、卵泡刺激素（FSH）等濃度，計算骨齡（BA）。結果 肥胖兒童血中leptin、Insulin濃度顯著(zhù)高于對照組濃度（P<0.01）；肥胖兒童血中orexinA濃度顯著(zhù)低于對照組濃度（P<0.01）；肥胖男生T顯著(zhù)低于對照組濃度，肥胖女生E₂、LH顯著(zhù)高于對照組濃度（P<0.01）；男女肥胖兒童骨齡明顯大于對照組，且隨著(zhù)骨齡的增大血中Leptin, LH含量明顯增加；男女肥胖與正常兒童LH與FSH之間、leptin與insulin, C-肽, E₂之間均存在正相關(guān)關(guān)系P<0.01；對照組男生LH, FSH與E₂存在正相關(guān)關(guān)系（r=0.373，0.314，P<0.05）；leptin與T存在負相關(guān)關(guān)系（r=-0.423，P<0.01），與FSH存在正相關(guān)關(guān)系（r=0.308，P<0.05）；對照組女生leptin, LH與E₂均存在正相關(guān)性（r=0.585, 0.647，P<0.01）；男女肥胖組LH、FSH與T、E2之間均未發(fā)現相關(guān)關(guān)系，肥胖男生leptin與T呈負相關(guān)關(guān)系（r=-0.574，P<0.01）。結論  肥胖兒童相對與正常兒童血中leptin、orexinA、insulin和T、E₂、LH濃度發(fā)生了明顯變化，肥胖兒童高濃度leptin水平可使體內T, E₂, LH濃度水平發(fā)生變化，影響肥胖兒童的青春期發(fā)育。

關(guān)鍵詞：食欲素  瘦素  胰島素  睪酮   雌二醇   黃體生成素  肥胖

 

Lou Xiao-min, Duan Guang-cai, Chen Jiang, Zhou Yan,  et al.

West China School of Public Health, Sichuan University, Chengdu  610041, China

 

Abstract : Objective  To investigate the level changes of obesity-associated factors and puberty-associated hormones and their relationship between obese and normal Children. Methods The concentrations of orexins, leptin, insulin, testosterone(T), estradiol(E₂), luteotrophic hormone(LH), as well as Follicle stimulating hormone(FSH) in blood were measured respectively in 78 cases of 13 years old obesity children and 84 normal children as a control group by the means of RIA methods. The bone ages were evaluated with “bone age standard of hundred percent scale”. Results  The leptin and insulin concentrations were significantly higher in obesity children as compared with the control group （P<0.01）. The concentrations of plasma orexinA were significantly lower in obesity children as compared with the control group（P<0.01）. The concentrations of T were significantly lower in obesity boys as compared with the control group （P<0.01）. The concentrations of E₂ and LH were significantly higher in obesity girls as compared with the control group （P<0.01）. The bone age of the obese children was significantly older than that of the control group. Along with the bone age of children became older, the concentration of leptin and LH in blood were significantly increased; There was a positive correlation between LH and FSH in two groups of either boys and girls and so did between leptin and Insulin, C- peptide, E₂. P<0.01；In the group of normal boys, the positive correlations between LH, FSH and E₂ were shown（r=0.373，0.314，P<0.05）, and a negative correlations between leptin and T were shown as（r=-0.423，P<0.01）,a positive correlation between leptin and FSH was also shown as （r=0.308，P<0.05）. In the control group of girls, there were positive correlations between leptin, LH and E₂（r=0.585，0.647，P<0.01）. Any correlations between LH, FSH and T, E₂ were not shown in the two obese groups of boys and girls. Conclusion  The leptin, orexinA, Insulin, T, E₂, and LH concentrations had been changed significantly in blood among those obesity children, and so as their interactions. The high level of leptin in obesity children may change the concentrations of T, E₂ and LH and affect the development of puberty.

Key words :orexins，leptin，insulin，luteotrophic hormone，testosterone，estradiol，obesity

 

 

基金項目：河南省自然科學(xué)基金項目（No.511041400）

作者簡(jiǎn)介：婁曉民，男，博士研究生，副教授，工作單位為鄭州大學(xué)公共衛生學(xué)院

1 鄭州大學(xué)公共衛生學(xué)院