孫長(cháng)顥  周曉蓉  趙丹

哈爾濱醫科大學(xué)公共衛生學(xué)院，哈爾濱  150086

 

摘要：目的  通過(guò)研究共軛亞油酸(CLA)對飲食誘導胰島素抵抗大鼠脂肪酸連接蛋白（ap2）基因表達的影響，探討CLA抗糖尿病作用的機制。方法  選用雄性Wistar大鼠，隨機分為對照組、高脂組、高脂+CLA組（每100g飼料含CLA分別為0.75g、1.50g、3.00g），每組動(dòng)物10只，觀(guān)察CLA對胰島素抵抗大鼠胰島素、血糖水平的影響，并應用RT-PCR的方法檢測ap2、過(guò)氧化物酶體增殖物活性受體γ（PPARγ）的表達水平。結果  高脂組大鼠血清游離脂肪酸（FFA）、胰島素和糖血水平顯著(zhù)高于基礎組，CLA 可降低胰島素抵抗大鼠血清FFA、血糖、胰島素水平，并可增加其脂肪組織ap2、PPARγ mRNA的表達水平。結論  CLA可通過(guò)激活PPARγ上調脂肪酸連接蛋白基因的表達，改善肥胖大鼠的胰島素抵抗。

關(guān)鍵詞：脂肪酸連接蛋白  過(guò)氧化物酶體增殖物激活受體γ  胰島素抵抗

 

School of Public Health, Harbin Medical University,Harbin 150086,China

 

Abstract:  Objective  To study the effect of conjugated linoleic acid on gene expression of fatty acid binding proteins（ap2）in white obesity tissue of  rats with insulin resistance, and explore the mechanism of resisting diabetes by CLA. Methods  Male Wistar rats were randomly separated to control group, high-fat group and high fat + CLA group(0.75g,1.50g,3.00g% by diet weight), Using reverse transcription polymerse chain reaction (RT-PCR) technique to measure the expression level of ap2 and Peroxisome proliferators-activated receptor－γ(PPARγ) mRNA expression. Results  The FFA, serum insulin and glucose levels of rats with insulin resistance were higher than that of rats fed with basic diet, supplement of CLA can decrease FFA, serum insulin and glucose levels of rats with insulin resistance, and CLA can  increase the expression of ap2 and PPARγ mRNA in adipose tissue of rat with insulin resistance. Conclusion  CLA can improve insulin resistance of obese rat and increase the expression of ap2 mRNA ,possibly acting through activing PPARγ.

Key words: ap2，PPARγ，insulin resistance

 

 

基金項目：國家自然科學(xué)基金資助項目（No.30271118）

作者簡(jiǎn)介：孫長(cháng)顥，男，教授，博士研究生導師，哈爾濱醫科大學(xué)公共衛生學(xué)院院長(cháng)

中國疾病預防控制中心營(yíng)養與食品安全所，北京  100050

 

摘要：目的 了解2000年中國不同地區膳食中總汞含量和成年男子人均汞膳食攝入量。方法采用總膳食研究方法，通過(guò)膳食調查、統計不同地區人群各種食物的消費量、按照所得的食物消費量數據烹調加工成可食的各種食物類(lèi)別。膳食樣品中總汞含量采用冷原子吸收光譜法測定，并按成年男子各種食品的消費量與實(shí)測膳食中汞的含量相乘得到不同地區總汞的實(shí)際攝入量。采用世界衛生組織推薦的總汞每周可耐受攝入量（PTWI）值評價(jià)中國四個(gè)大區成年男子膳食中總汞攝入的安全性。結果 除某些地區的個(gè)別食物樣品超過(guò)中國食品衛生中總汞限量標準外，四個(gè)大區和全國成年男子平均膳食總汞攝入量（占%PTWI）分別為：8.86µg (19.7%)、4.69µg (10.4%)、7.01µg (15.6%)、6.88µg (15.3%)和6.86µg (15.2%)。結論  結果顯示中國人群膳食中總汞攝入量是安全的。

關(guān)鍵詞：中國總膳食研究  膳食汞攝入量  膳食營(yíng)養

 

National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention，Beijing 100050, China

 

Abstract：Objective  To assess the safety of dietary mercuric intakes in different areas in China. Methods  Using the Chinese total dietary study method, the analytical samples were obtained in different areas by food consumption survey, food aggregation, food sampling and preparation. The levels of dietary total mercury were determined by cold vapor atomic absorption spectrometry. The dietary total mercuric intakes in different areas were obtained by timing the food consumption data and the mercuric content in different dietary samples. The safety of dietary mercury was evaluated in Chinese four regions and average adults by using the dietary mercuric PTWI recommended by WHO. Results  The results indicate that the dietary mercuric intakes are safe in different regions. Only a few samples in some areas exceed tolerance limits of China national standard. Dietary total mercuric intakes (of PTWI) in four different regions adults and mean were 8.86µg (19.7%), 4.69µg (10.4%), 7.01µg (15.6%), 6.88µg (15.3%), 6.86µg (15.2%), respectively. Mainly sources of dietary mercuric intake were cereals, vegetables and sea foods. Conclusion  The result shows that the total mercuric intakes in Chinese people are safe.

Key words: Chinese total diet study, dietary mercuric intakes

 

 

 

基金項目：科學(xué)技術(shù)部科技基礎性工作項目基金

作者簡(jiǎn)介：李筱薇，女，助理研究員，中國協(xié)和醫科大學(xué)公共衛生學(xué)院碩士研究生
文章編號：1000-8020（2006）03-0357-03

劉跟生　徐貴發(fā)

山東大學(xué)公共衛生學(xué)院營(yíng)養與食品衛生研究所，濟南  250012

摘要：單不飽和脂肪酸（MUFA）是脂肪酸的一種，其碳鏈上只有一個(gè)雙鍵。研究發(fā)現MUFA能夠正向調節血脂代謝，降低低密度脂蛋白膽固醇（LDL）的氧化敏感性，保護血管內皮和降低血液高凝狀態(tài)。本文將從以上幾個(gè)方面介紹MUFA對心血管的保護作用。

關(guān)鍵詞：單不飽和脂肪酸  血脂  內皮  心血管

 

Astract: Monounsaturated fatty acid （MUFA） is a sort of fatty acids, including a double bond in its carbon chain. Substantial evidence exists to suggest that MUFA can positively regulate the metabolism of serum lipids, decrease the susceptibility of LDL to oxidative modification, protect the artery endothelium, and decrease prothrombotic environment. The protective effect  of MUFA to the cardiovascular system was introduced in the paper.

Key words：monounsaturated fatty acid, serium lipids, endothelium, cardiovascular system

 

作者簡(jiǎn)介：劉跟生，男，碩士研究生，E-mail：nutritiongen@163.com